Classroom Kit – Restriction Enzyme Mapping – For 6 Sets of Restriction Digestions

ABOUT THIS PRODUCT:
In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.

Group Size:
6 sets of restriction digestions

Time Required:
Complete in 1 hour 20 minutes to 1 hour 45 minutes

Kit Includes:
Instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain® Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps

All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, UV Transilluminator (for InstaStain™ Ethidium Bromide), White Light Box (for FlashBlue™), & Microwave or Hot plate.

Storage:
Some Components Require Freezer Storage Upon Receipt