Classroom Kit – Lighting Up Life: Expression of GFP in C. elegans – For 10 Groups

ABOUT THIS PRODUCT:
Scientists can directly manipulate an organism’s genome to produce a phenotype using engineered genes called transgenes. In this experiment, students will use fluorescent microscopy and PCR to analyze C. elegans (nematodes) that have been engineered to express the Green Fluorescent Protein (GFP).

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

Group Size:
For 10 lab groups

Time Required:
Growing Bacteria – Overnight
Plating Worms – 15 minutes
Worm Growth – 3-4 Days
Set Up – 30 minutes
PCR – 2 hours or overnight
Electrophoresis – 90 minutes

Kit Includes:
Instructions, C. elegans, E. coli OP50 cells, PCR EdvoBeads™ PLUS, GFP primer mix concentrate, EdvoQuick™ DNA ladder, control DNA concentrate, rehydration buffer, sterile water, TE buffer, extraction buffer, ReadyPour™ NGM agar, NGM salts, petri dishes, pipets, tubes, UltraSpec-Agarose™, electrophoresis buffer (50x), 10x gel loading solution, SYBR® Safe DNA stain, FlashBlue™ liquid stain, sterile loops, & wax beads

All You Need:
Covered box, fluorescent and dissecting microscopes, timers, water bath, incubator (optional), thermal cycler or three water baths, electrophoresis apparatus, power supply, balance, microcentrifuge, UV transilluminator or UV photodocumentation system, automatic micropipettes with tips, microwave or hot plate, pipet pump, permanent markers, flasks or beakers, hot gloves, distilled or deionized water, sterile water, ice buckets and ice, bleach solution or laboratory disinfectant

Storage:
Some Components Require Refrigerator & Freezer Storage