Classroom Kit – Immunoelectrophoresis – For 10 separations

ABOUT THIS PRODUCT:
Learn how immunoelectrophoresis identifies proteins based on their combined electrophoretic and immunological properties. This method is useful to monitor antigen and antigen-antibody purity and to identify a single antigen in a mixture of antigens. In this experiment, serum proteins are separated by agarose gel electrophoresis and the point of equivalence is observed by the antigen-antibody complex formation.

Group Size:
For 10 separations

Time Required:
Electrophoresis – 60 minutes / Incubation – overnight

Kit Includes:
Instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipets, well cutters, tubes, filter paper, 10mL pipettes, & 60mm petri plates.

All You Need:
Horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, microscope slides (1″ x 3″), distilled water, 400-600 mL beaker, 1000 mL graduated cylinder, microwave, humidifying chamber, paper towels, plastic wrap or foil, & 37˚C incubation oven (optional).

Storage:
Refrigerator