Classroom Kit – Effects of Alcohol on C.elegans – For 10 Groups

ABOUT THIS PRODUCT:
You will not believe how similar we are to worms! The genome of the tiny worm, Caenorhabditis elegans, was sequenced and found to be 40% similar to us. It is now used as a model system by researchers to address fundamental questions in developmental biology, neurobiology and behavioral biology. The objective of this experiment is to observe and record the effects of alcohol on normal and alcohol mutant strains of Caenorhabditis elegans.

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

Group Size:
For 10 lab groups

Time Required:
Growing Bacteria – overnight
Plating Worms – 15 minutes
Worm Growth – 3-4 days
Alcohol Experiment – 45-60 minutes

Kit Includes:
Instructions, C. elegans-normal, C. elegans Alcohol-resistant, E.coli OP50 BactoBeads™, S-Buffer, 10% Alcohol, ReadyPour NGM Agar, NGM Medium Salts, Petri Dishes, Large/Small Transfer Pipets, 1.5 ml Microcentrifuge Tubes, Cell Counting Chamber, Sterile Loops, 15 ml centrifuge tubes, and 10 ml pipet

All You Need:
Covered box, microscopes, timers, 70% ethanol, water bath, and incubator (optional).

Storage:
Some Components Require Refrigerator Storage