Classroom Kit – Environmental Toxicity Response in C.elegans – For 10 Groups

ABOUT THIS PRODUCT:
Caenorhabditis elegans is a soil nematode with great potential for educational research, partly because of its rapid (3-day) life cycle, small size (1.0-mm-long adult), and ease of laboratory growth cultivation. In this experiment, students will observe and compare the effects of heavy metals found in the environment on normal and mutant strains of Caenorhabditis elegans (C. elegans).

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

Group Size:
For 10 lab groups

Time Required:
Growing Bacteria – overnight
Plating Worms – 15 minutes
Worm Growth – 3-4 days
Toxicity Experiment – 45-60 minutes

Kit Includes:
Instructions, C. elegans-normal, C. elegans-Toxicity mutant, petri dishes, NGM medium salts, ReadyPour NGM Agar, E. coli 0P50 Bactobeads™, cell counting chambers, buffer, pipets, sterile loops, tubes and heavy metal compounds.

All You Need:
Ethanol, timers, microscopes, water bath, covered box, incubator (optional).

Storage:
Some Components Require Refrigerator Storage.