Classroom Kit – Fingerprinting of Bacterial Proteins – For 6 Groups sharing 3 gels

ABOUT THIS PRODUCT:
The objective of this experiment is for students to prepare soluble protein lysates from several species of bacteria and analyze the electrophoretic protein profiles to identify an unknown.

Group Size:
For 6 groups sharing 3 gels

Time Required:
Day One Set Up – 15 minutes / Day Two Set Up – 60 minutes / Electrophoresis – 60 minutes / Staining – 20 minutes / Destaining – 2 hours

Kit Includes:
Instructions, bacterial cultures and reagents, Lysozyme, buffers, Protein InstaStain®, practice gel loading solution, protein sample buffer, unknown proteins ready for electrophoresis, ReadyPour™ Agar, nutrient broth, screw cap tubes, sterile inoculating loops, sterile 1 ml pipets, sterile petri plates, 100 x 15 mm

All You Need:
3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, microcentrifuge, incubation oven, hot plate or burner, white light visualization system, automatic micropipette with fine tips, lab glassware, methanol, glacial acetic acid, distilled water, sterile water or nutrient broth, plastic wrap, photodocumentation system (optional)

Storage:
Some Components Require Freezer Storage