Classroom Kit – Separation of RNA and DNA by Gel Filtration Chromatography – For 5 Separations and 5 Gels

ABOUT THIS PRODUCT:
Gel filtration chromatography separates molecules on the basis of size and shape. This experiment provides a LyphoSample™ mixture of RNA and DNA that is separated on a gel exclusion column. The purified fractions of DNA and RNA are analyzed by agarose gel electrophoresis.

Group Size:
For 5 separations and 5 gels

Time Required:
Complete in 90 minutes

Kit Includes:
Instructions, DNA/RNA LyphoSample™, Dry matrix, elution buffer, chromatography columns, agarose, gel loading solution, buffer, InstaStain® Blue and FlashBlue™ stain.

All You Need:
Electrophoresis apparatus, power supply, ring stands with clamps, automatic micropipette with tips, microwave or hot plate, pipet pumps or bulbs, 5 or 10 ml pipets, balance, lab glassware, weigh boats, white light visualization system, photodocumentation system (optional), distilled water.

Storage:
Refrigerator