Classroom Kit – Southern Blot Analysis – For 5 Groups

ABOUT THIS PRODUCT:
This experiment introduces your students to Southern blotting as a tool for “DNA Fingerprinting” in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining.

Group Size:
For 5 lab groups

Time Required:
Electrophoresis – 45 minutes / Blot – overnight / Stain/Destain – 10 minutes

Kit Includes:
Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™.

All You Need:
Electrophoresis apparatus, power supply, 65º C Water bath, DNA visualization system, staining net & tray, automatic micropipettes, lab glassware, microwave or hot plate, distilled water, NaCl, NaOH, concentrated HCl, plastic wrap, forceps