Classroom Kit – Cleavage of Lambda DNA with EcoRI Restriction Enzyme – For 10 Restriction Digestions and 5 Gels

ABOUT THIS PRODUCT:
The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.

Group Size:
For 10 restriction digestions and 5 gels

Time Required:
Complete in 90 minutes

Kit Includes:
Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK® Enzyme Grade Water, Eco RI Dryzymes™ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template.

All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, White Light Box, & Microwave or Hot Plate.

Storage:
Some Components Require Freezer Storage Upon Receipt