Classroom Kit – Rainbow Transformation – For 10 Groups

ABOUT THIS PRODUCT:
In this colorful experiment, students will explore the biological process of bacterial transformation using vibrant chromogenic proteins. Students will use three recombinant plasmids to transform E.coli bacteria. The resulting E.coli will be examined for the presence of blue, purple, and pink pigments, as well as for resistance to ampicillin. Features our new enhanced transformation protocol for improved student results.

Group Size:
For 10 lab groups

Time Required:
Set Up & Plating – 50 minutes / Incubation – overnight / Transformation efficiency – 15 minutes

Kit Includes:
Instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ Luria Broth Agar, sterile, recovery broth, petri plates, sterile pipets, loops and microtubes.

All You Need:
Adjustable volume micropipette (5-50µl and 50-200µl recommended) and tips, two water baths (37˚C and 42˚C), thermometer, incubation oven (37˚C), centrifuge, pipet pumps or bulbs, ice and ice bucket, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves

Storage:
Some components require refrigerator and freezer storage