Classroom Kit – What’s In My Lunch? Quantitative Food Allergy ELISA – For 10 Groups

ABOUT THIS PRODUCT:
NGSS-aligned with MS-LS1

Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods. In this inquiry-based experiment, students will master the concepts behind the enzyme-linked immunosorbent assay (ELISA). Students will perform an ELISA to detect the presence and measure the concentration of whey protein in various food products.

Group Size:
For 10 lab groups

Time Required:
Complete in 2 horus

Kit Includes:
Instructions, 10x ELISA wash buffer, ELISA dilution buffer, whey antigen (lyophilized), anti-whey antibody (lyophilized), secondary antibody, TMB substrate, stop solution, strip tubes (8-well), snap-top microcentrifuge tubes, homogenization pestles with tubes, 15 mL conical tubes, & transfer pipets

All You Need:
Various food samples to be tested, distilled or deionized water, beakers or flasks, paper towels, disposable lab gloves, safety goggles, automatic micropipettes (5-50µl, 100-1000µl) and tips, digital camera or cell phone with camera (optional), computers with internet, image analysis program, and graphing program (optional)

Storage:
Some Components Require Refrigerator Storage