Classroom Kit – AIDS Kit I: Simulation of HIV Detection by ELISA – For 10 Groups

ABOUT THIS PRODUCT:
An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient’s blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a color change upon addition of substrate. In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Group Size:
For 10 lab groups

Time Required:
Complete in 60 minutes

Kit Includes:
Instructions, 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary antibody (lyophilized), secondary antibody (lyophilized), ABTS (lyophilized), ABTS reaction buffer, microtiter plates, transfer pipets, snap-top microcentrifuge tubes, 15 mL conical tubes

All You Need:
Distilled or deionized water, beakers, disposable lab gloves, safety goggles, automatic micropipettes (50µl) and tips (recommended)

Storage:
Refrigerator