Classroom Kit – In Search of the Sickle Cell Gene by Southern Blot – For 5 Groups

ABOUT THIS PRODUCT:
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.

Group Size:
For 5 lab groups

Time Required:
Electrophoresis – 45 minutes | Blot – overnight | Stain & Destain – 10 minutes

Kit Includes:
Instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain.

All You Need:
Electrophoresis apparatus, power supply, microwave or hot plate, water bath and 80ºC incubation oven.

Storage:
Some Components Require Freezer Storage