Classroom Kit – VNTR Human DNA Typing Using PCR – For 25 Students

ABOUT THIS PRODUCT:
In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. In this kit, students will type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

Group Size:
For 25 students

Time Required:
Extraction – 30 minutes
PCR Setup – 10 minutes
PCR – 90 minutes
Electrophoresis – 30 minutes
Staining – 5 minutes

Kit Includes:
Instructions, PCR EdvoBeads™, LyphoPrimer™ mix, EdvoQuick™ DNA Ladder, LyphoControl™, Universal DNA Buffer, TE Buffer, Proteinase K, UltraSpec-Agarose™, TBE Electrophoresis Buffer Powder, SYBR® Safe Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & a 15 mL conical tube.

All You Need:
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (2) Water Baths, Microcentrifuge, Microwave or Hot plate.

Storage:
Some Components Require Freezer Storage Upon Receipt