Classroom Kit – Reverse Transcription PCR (RT-PCR) – The Molecular Biology of HIV Replication – For 6 Groups

ABOUT THIS PRODUCT:
A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory.
* Requires wet ice shipment for next day delivery (by 3:00 pm in most areas)

Group Size:
For 6 lab groups

Time Required:
PCR Setup – 10 minutes
PCR – 3 hours
Electrophoresis – 60 minutes
Staining – 5 minutes

Kit Includes:
Instructions, RNA LyphoTemplate™, LyphoPrimer™ mix, tubes with RT-PCR EdvoBeads™, RNAse-free water, EdvoQuick™ DNA Ladder, UltraSpec-Agarose™, 50X Electrophoresis Buffer, 10X Gel Loading Solution, SYBR® Safe stain, microcentrifuge & PCR tubes.

All You Need:
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, (3) Water Baths (if performing experiment without a thermal cycler), Microcentrifuge & Microwave or Hot plate.

Storage:
Some Components Require Freezer Storage Upon Receipt