Classroom Kit – Chemotaxis: The Science of Attraction in C.elegans – For 10 Groups

ABOUT THIS PRODUCT:
All organisms are affected by “scent” molecules in the environment, including a multicellular organism called Caenorhabditis elegans. These worms are composed of 959 somatic cells, of which 300 are neurons comprising organs for taste, smell, temperature and touch. In this experiment, your students will observe and record the phenomenon by which normal and mutant strains of C. elegans can direct their movement in response to certain chemicals in the environment.

*Kit contains LIVE materials. Please request materials 2 weeks prior to lab*

Group Size:
For 10 lab groups

Time Required:
Growing Bacteria – overnight
Plating Worms – 15 minutes
Worm Growth – 3-4 days
Chemotaxis – 45-60 minutes

Kit Includes:
Instructions, C. elegans – normal, C. elegans Chemotaxis – mutant, petri dishes, NGM medium, E. coli OP50 BactoBeads™, cell counting chambers, buffer, pipettes, sterile loops, tubes and chemical compounds.

All You Need:
Ethanol, timers, microscopes, covered box, water bath, incubator (optional).

Storage:
Some Components Require Refrigerator Storage