Classroom Kit – The Dose Makes the Poison: Testing the Envir. Impacts of Pollution – For 10 Lab Groups

ABOUT THIS PRODUCT:
Biological assays, or bioassays, are powerful tools that allow scientists to determine the effects of a given substance on living organisms. In this inquiry-based lab, students plan and perform a plant bioassay to determine the environmental hazard of common point and non-point source pollutants. The results are analyzed using averages, standard deviations, and TC50 calculations, integrating STEM.

Group Size:
For 10 lab groups

Time Required:
2 hours, 15 minutes (plus 1 week growing)

Kit Includes:
Instructions, radish seeds, plant growth medium, antifungal powder, pollutant solutions, cheese cloth, petri dishes, transfer pipets, seriological pipet, conical tubes, microcentrifuge tubes, small loops.

All You Need:
Water bath, pipet pump, microwave or hot plate, 70% ethanol, sterile water, bleach, automatic micropipette and tips, parafilm, string, rulers, growth light (optional), analytical balance (optional).

Storage:
Some Components Require Refrigerator and Freezer Storage