Classroom Kit – Multiplex PCR Testing of Water Contaminants – For 25 Students

PRODUCT UPDATE:
Experimental components have changed to include new LyphoPrimer™ and LyphoControl™ reagents and SYBR® Safe DNA stain. Please read the accompanying literature as directed in your kit.

ABOUT THIS PRODUCT:
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Group Size:
For 25 students

Time Required:
Extraction – 60 minutes
PCR Setup – 10 minutes
PCR – 2 hours
Electrophoresis – 60 minutes
Staining – 5 mins or overnight

Kit Includes:
Instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR® Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes.

All You Need:
DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 µl, UV or Blue Light Transilluminator, Water Bath, Microcentrifuge, Microwave or Hot plate, & Isopropanol.

Storage:
Some Components Require Refrigerator or Freezer Storage Upon Receipt