Classroom Kit – DNA Damage and Repair – For 10 Groups

ABOUT THIS PRODUCT:
According to the World Health Organization, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cancers are caused by preventable damage to DNA by UV light during sunbathing. In this experiment, your students will expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analyzed by agarose gel electrophoresis to observe the damage.

Group Size:
For 10 lab groups

Time Required:
Complete in 2 hours

Kit Includes:
Instructions, standard DNA fragments, plasmid DNA, gel loading solution, agarose, electrophoresis buffer, 1 ml pipet, microtest tubes, 100 ml graduated cylinder, InstaStain® Ethidium Bromide stain.

All You Need:
UV transilluminator (254 nm or short wave), electrophoresis apparatus, power supply, microwave or hot plate.

Storage:
Some Components Require Freezer Storage